Cardioprotective effect of ellagic acid on doxorubicin induced cardiotoxicity in wistar rats

Abstract

Background/Aim: The objective of the study was to evaluate the cardioprotective activity of ellagic acid in doxorubicin induced cardiotoxicity. Methods: Male wistar rats (180-220g) were divided in to four groups: vehicle control, doxorubicin control, ellagic acid (100mg/kg) and ellagic acid (200mg/kg). Doxorubicin was administered to all the groups except vehicle control at a dose of 3.75mg/kg at week 2,3,4,5 to make a cumulative dose of 3.75mg/kg. Ellagic acid was administered to the respective group of animals at a dose of 100mg/kg 200mg/kg, respectively once daily orally for 6 weeks. On the last day of the study, blood was collected by retro-orbital puncture and LDH and CK-MB were estimated. The animals were cannulated and the ECG and hemodynamic parameters were recorded. The animals were then sacrificed and histology of heart was performed. Results: Doxorubicin showed cardiotoxicity manifested by changes in serum marker enzymes, ECG and hemodynamic parameters which was further confirmed by histology of heart. This doxorubicin induced changes were attenuated by treatment with ellagic acid (200mg/kg).Conclusion: It has been concluded that ellagic acid (200mg/kg) treatment for 6 weeks protects the heart of rats in doxorubicin induced cardiotoxicity.