Rapid lipid content screening in neochloris oleoabundans utilizing carbon-based dielectrophoresis

Abstract

In this study, we carried out a heterogeneous cytoplasmic lipid content screening of Neochloris oleoabundans microalgae by dielectrophoresis (DEP), using castellated glassy carbon microelectrodes in a PDMS microchannel. For this purpose, microalgae were cultured in nitrogenreplete (N+) and nitrogen-deplete (N−) suspensions to promote low and high cytoplasmic lipid production in cells, respectively. Experiments were carried out over a wide frequency window (100 kHz–30 MHz) at a fixed amplitude of 7 VPP. The results showed a statistically significant difference between the dielectrophoretic behavior of N+ and N− cells at low frequencies (100–800 kHz), whereas a weak response was observed for mid-and high frequencies (1–30 MHz). Additionally, a finite element analysis using a 3D model was conducted to determine the dielectrophoretic trapping zones across the electrode gaps. These results suggest that low-cost glassy carbon is a reliable material for microalgae classification—between low and high cytoplasmic lipid content—through DEP, providing a fast and straightforward mechanism.