Techniques microbiolization seed forage radish with Trichoderma spp. and Bacillus subtilis

Abstract

The objective of this study was the effect of leverage microbiolization forage radish seed with Trichoderma spp. and Bacillus subtilis by physiological conditioning techniques, suspension of biological structures and film coating. The microbiolization suspension of biological structures was carried out with commercial products Rhizoliptus® and Agrotrich plus®. Fluid restriction was held in PDA medium + mannitol (-0.7 MPa), when was the first seed radicle protrusion in the other were removed and dried in a laboratory environment for 48 h. The film coating was performed with the addition of the polymer Color Seed® (300 mL kg-1) treatment of the syrup containing Trichoderma spp. or product Rhizoliptus®. We used a treatment covering the seeds primed with organisms, or their association. The microbiolization with Trichoderma spp. by physiological conditioning controls pathogens infecting the seeds. Trichoderma spp. and Bacillus subtilis promote the growth and development of seedlings of forage radish, and the coating of the seeds primed in the presence of organisms which provides greater shoot growth of seedlings in the field.