Growth of mastigocladus (Cyanobacteria) HS-46 isolated from maribaya hot spring, Indonesia in medium NPK as a potential biofuel feedstock

Abstract

One of cyanobacteria genera which isolated from Maribaya hot spring located in Indonesia is Mastigocladus HS-46 can be used as raw material for biofuel production. Mastigocladus is cultured in commercial NPK fertilizer. Commercial NPK fertilizer is one of the low cost medium that commonly used for microalgae growth. The research aims to know the best concentration of commercial NPK fertilizer for Mastigocladus HS-46 growth. In this research, Mastigocladus HS-46 was grown in commercial NPK fertilizer with concentration 80 ppm, 240 ppm, NPK 80 ppm+BBM, and BBM (Bold Basal's Medium) as a control. This strain was incubated on 35°C. Observation were made approximately 14 days with 2 sampling for each medium. The average of wet weight Mastigocladus HS-46 after 14 day observation was obtained at NPK 80 ppm: 0,019 g/L, NPK 240 ppm: 0.009 g/L, NPK+BBM: 0,014 g/L, and BBM: 0,015 g/L. The results showed that the best NPK medium concentration was 80 ppm for growth Mastigocladus HS-46. Commercial NPK fertilizer can be used for growth Mastigocladus HS-46 which has potential as biofuel feedstock.