Application of ellipsometry, spr-technic and raman-spectroscopy into diagnosis of colorectal cancer

Abstract

Experiments with application of the "Ellips-SPEK" spectral ellipsometric SPR (Surface Plasmon Resonance) analyzer, validating the results on the basis of ProteOn XPR36, revealed the differences in the values of velocity constants of specific interactions of monoclonal highly specific antibodies with tumor M2-pyruvate kinase on the surface of blood serum biochips in patients with various stages colorectal carcinoma (CRC) and location of metastases (AUC 0.89). Scanning ellipsometry enabled to reveal a significant increase of biomolecule surface effective thickness resulting from the specific "antigen-antibody" interaction in the group of patients with extrahepatic and hepatic metastases, as compared to the control group with healthy patients (p<0.0001-0.05). Raman spectroscopy of blood serum demonstrated the differences in peak intensity within the range of 1005-1520 cm-1 in the same groups of patients (p<0.0001-0.05) with a 90% predictive accuracy of the method applied at early stages of the disease. The results obtained can be used for development of methods for early diagnosis of CRC, localization of different metastases, relapses and control of the quality of administered therapy.