In-vitro Comparison of Anti-urolithiatic Effect of Crataeva nurvala, Zea mays (Corn Silk) and Ayurvedic Formulation Neeri Tablet

Abstract

The main objective of this study was to determine the anti-urolithiatic activities of the C. nurvala bark, Zea mays (corn silk), and ayurvedic formulation Neeri tablet taking Cystone as a standard formulation. The in vitro anti-urolithiatic activity of the extracts was performed based on their ability to neutralize synthetic calcium oxalate crystals in a semi-permeable eggshell membrane. The quantity of undissolved calcium oxalate was determined titrimetrically based on the volume of standard potassium permanganate (KMnO4) consumed. Each mL of 0.9494 N KMnO4 is equivalent to 0.1898 mg of calcium, the percentage dissolution of calcium oxalate was calculated. The results showed that the aqueous extract of corn silk dissolved a 60.42 % calcium oxalate crystal, comparable to Ayurvedic formulation, Neeri, 59.52 % and standard Cystone 64.29 %, along with moderate effect of methanolic extract of corn silk, aqueous and methanolic C. nurvala extract. The study concluded the significant anti-urolithiatic activity of corn silk aqueous extract.