Molecular characterization of selected genera of the (Apiaceae) family using ssr molecular markers

Abstract

The conducted study which included the study of a few selected races from canopy plants from the chemical perspective which included Apium graveilens L, Anethum graveilens L, Ciriandrum sativum L, Petriselium crispum L, cyminum Cuminum L, Fieniculum vulgare L, Pimpinella anisum L, Daucus carrit L and Ammi majus L. The DNA was detected and extracted and Conducted molecular tests on the DNA using (SSR Simple Sequence Repeats) using the polymerization device (PCR) and electrophoresis process on Agarose Gel. Five selected and specific prefixes were used which approved its effectiveness in giving multiple shapes (Polymorphic) among the studied plants of 95%, as packs were obtained from a total number of 14 out of 53 different pack with an arithmetic average of 2.8 for each prefix. Cluster analysis and phylogenetic tree have shown that there is a genetic affinity between 0.84615-0.125 according to the two variables data (1-0) the genetic similarity using cluster analysis (UPGMA)has allowed to draw the portfolio diagram among the studied races, It can be said that the SSR Simple Sequence Repeats can be an effective technique for studying molecular characterization and can be used to separate the genetic relations in the plant world.