P-class pentatricopeptide repeat proteins are required for efficient 5′ end formation of plant mitochondrial transcripts

Abstract

It is well recognized that flowering plants maintain a particularly broad spectrum of factors to support gene expression in mitochondria. Many of these factors are pentatricopeptide repeat (PPR) proteins that participate in virtually all processes dealing with RNA. One of these processes is the post-transcriptional generation of mature 5′ termini of RNA. Several PPR proteins are required for efficient 5′ maturation of mitochondrial mRNA and rRNA. These socalled RNA PROCESSING FACTORs (RPF) exclusively represent P-class PPR proteins, mainly composed of canonical PPR motifs without any extra domains. Applying the recent PPRnucleotide recognition code, binding sites of RPF are predicted on the 5′ leader sequences. The sequence-specific interaction of an RPF with one or a few RNA substrates probably directly or indirectly recruits an as-yet-unidentified endonuclease to the processing site(s). The identification and characterization of RPF is a major step toward the understanding of the role of 5′ end maturation in flowering plant mitochondria. © 2013 Landes Bioscience.