Decrease in particle-induced osteolysis in ovariectomized mice

Abstract

Postmenopausal osteoporosis is a common disorder that results from increased osteoclastic activity caused by estrogen deficiency. Whether postmenopausal bone remodeling can alter the response to particulate debris is unknown. The purpose of this study was to evaluate the bone response to polyethylene particles in an ovariectomized murine model. Polyethylene particles were implanted onto the calvaria of seven control mice and seven ovariectomized (OVX) mice, as compared with calvaria from sham-operated andOVXmice. Calvaria were harvested after 14 days. Skulls were analyzed with a high-resolution micro-CT and by histomorphometry after staining with Stevenel blue and picrofuschine, and for tartrate-specific alkaline phosphatase. As assessed by micro-CT, particle implantation induced a significant decrease in bone thickness in control mice, while bone thickness remained stable in OVX mice. In particle-implanted animals, the osteoclast number was 2.84±0.3 in control mice and 1.74±0.22 in OVX mice. Mean bone loss was -12%±1.9% in control mice and -4.7%±1.7% in OVX animals. The reduction of osteolytic response suggests that ovariectomy may have a protective role against particle-induced bone resorption. © 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.