Viability of biofertilizer bacteria Rhizobium spp based on household waste

Abstract

The research was carried out from May 2021 to August 2021. The research was carried out at the Jember State Polytechnic Plant Protection Laboratory. This study used four types of household waste media, namely rice washing water, rice bran water, soybean dregs water, and Yeast Mannitol Agar media as controls. Each treatment was repeated 5 times to obtain 20 experimental units. The data obtained were then analyzed qualitatively and quantitatively. Quantitative data analysis by calculating the number of colonies with TPC (total plate count) and T-test techniques, qualitative data obtained by observing bacterial purity and gram staining. The results of this study indicate that alternative media for bran water waste conventionally produced the highest bacterial population with a colony number of 6,80 x 1024 CFU and the lowest bacterial population came from rice alternative media with a colony number of 1.28 x 1024 CFU. Bacterial viability observed for 4 months between aseptic media and conventional media showed the results of the t-test with a count of 1.49 x 10-1 CFU. shows the results (ns) are not significantly different.