Detection of naturally occurring alloantibody by an in-clinic antiglobulin-enhanced and standard crossmatch gel column test in non-transfused domestic shorthair cats

Abstract

Background: Blood typing for the A and B antigens is essential and crossmatching testing is generally recommended before transfusing blood to cats. Objective: To evaluate 2 crossmatch (XM) tests. Animals: Forty-nine healthy domestic shorthair cats that had not received a blood transfusion. Methods: Prospective study. Blood samples were typed for AB using immunochromatographic and flow cytometric techniques. A gel column (GC) and a feline antiglobulin-enhanced gel column (AGC) XM tests were used for crossmatching. Results: The population included 34 type A, 13 B, and 2 AB cats, with concordant results (r = 1, P