Regulation of the temperature-dependent activation of transient receptor potential vanilloid 1 (TRPV1) by phospholipids in planar lipid bilayers

Abstract

Background: Regulation of the temperature-dependent activity of TRPV1 channels with phosphoinositides is not well understood. Results: Phosphoinositides directly facilitate heat activation of TRPV1 reconstituted in neutral planar lipid bilayers. Different anionic lipids evoke TRPV1 channel activity with distinct open probability and conductance states. Conclusion: Phospholipids are required for heat-induced activation of TRPV1. Significance: Phosphoinositides are essential regulators of TRPV1 gating.