Prevalence, and virulence determination of listeria monocytogenes strains isolated from clinical and non-clinical samples by multiplex polymerase chain reaction

Abstract

Introduction: This study aimed to determine the prevalence, and virulence factors of Listeria monocytogenes isolated from various samples by multiplex polymerase chain reaction (MPCR). Methods: A total of 617 isolates were obtained and MPCR was employed for detection of the inlA, inlC, and inlJ genes. Results: L. monocytogenes was detected in 46 (7.45%) of the 617 specimens. inlA, inlC, and inlJ were detected in 100%, 76.26%, and 71% isolates, respectively. Conclusions: This study validated MPCR in the analysis and rapid detection of L. monocytogenes. The role of the genes in pathogenesis of the strains can also be affirmed.