Genetics of biotin biosynthesis in Bacillus subtilis

Abstract

Biotin auxotrophs of B. subtilis were isolated and classified into three groups according to growth requirements, cross feeding pattern, and biotin precursors excreted into culture supernatant fluids. Mutant genes were mapped by transduction using phage PBS1. All presently identified bio genes were linked to aroG with an order of bio aroG argA leu 1. No linked markers were found to the left of the bio loci.