Induction of metallothioneins in Mullus barbatus as specific biomarker of metal contamination: A field study in the western Mediterranean

Abstract

Red mullet (Mullus barbatus, Linnaeus, 1758) is one of the fish species used as bioindicator in the Mediterranean Pollution Monitoring Programme (MEDPOL). This paper presents the results of a field study on the induction of hepatic metallothioneins (MT) in red mullet as specific biomarker of metal contamination. Specimens of M. barbatus were collected in six areas with different degrees of anthropogenic activities along the Iberian Mediterranean coast. The environmental exposure to heavy metals of the populations was determined through the analyses of surface sediments collected in each study area. The MT levels obtained showed statistically significant differences between areas, but not between specimens of different sex. Furthermore, the highest MT levels were found in the most polluted areas. Hepatic MT levels in red mullet reflect the metal contamination present in surface sediments, especially for Cu, for which a significant linear regression was found. The MT levels are better correlated with Cu and Cd concentrations than with Zn and Hg concentrations. The MT values obtained in this study are the first to be reported for this species in the Iberian Mediterranean and they should help in the interpretation of MT levels and to establish MT background levels for M. barbatus in the western Mediterranean.