Production and characterization of exopolysaccharide from marine Bacillus sp. MSHN2016 with studying its effect on isoniazid/ rifampicin-induced hepatic and renal toxicities in rats

Abstract

Exopolysaccharides (EPSs) are bioactive natural products used in different applications. A preliminary chemical analysis of EPSMSHN indicated that the monosaccharides were arabinose, xylose, glucose and glucuronic acid with a relative ratio of 1: 1: 2: 1, respectively, with a weight average molecular weight (Mw) of 5.50 × 105 g/mol and a number average molecular weight (Mn) of 3.45 × 105 g/mol. INH/RIF deteriorated hepatic and renal functions through decreased hepatic and renal GSH and increasing MDA contents that associated with hepatocellular apoptosis as well as degeneration and fibrosis in many tubules. The treatment with EPSMSHN at different doses (50 and 100 mg/kg) corrected all previously mentioned INH/RFP-induced changes. In conclusion, EPSMSHN at both doses protected from the hepatic and renal toxicity induced by INH/RFP through its antioxidant and antifibrotic influence.