Determination of Pyrazinamide in Human Plasma Samples Containing Fixed Dose Combination Molecules by using Liquid Chromatography Tandem Mass Spectrometry

Abstract

A rapid, simple, sensitive and compatible liquid chromatography tandem mass spectrometric method has been developed and validated for the estimation of Pyrazinamide. Glipizide was used as an internal standard. Detection was performed using TSQ Quantum Discovery max spectrometer wth ESI source in positive polarity. The separation of analyte and internal standard were carried out using a reverse hase colunm....