Histological and cd31 immunolocalization after pulp therapy using mta or portland cement

Abstract

In cases of deep caries or trauma of primary teeth, pulpotomy is the procedure of choice to preserve pulp vitality and the teeth. This study aimed to evaluate the histological analysis and CD31 immunolocalization after pulpotomy using Mineral Trioxide Aggregate or Portland cement in human primary teeth. Twenty mandibular primary molars were divided into two study groups: G1-Mineral Trioxide Aggregate and G2-Portland cement. After conventional pulpotomy, clinical and radiographic follow-up, teeth at the regular exfoliation period were extracted for histological analysis and CD31 immunolocalization. The statistical analyses were performed by Kruskal-Wallis followed by Dunn test (P < 0.05). No statistically difference regarding inflammation and blood vessels amount (P > 0.05) were found between groups. The immunohistochemistry analysis revealed positive CD31 expression in the blood vessels of both studied groups. Group 1 showed mainly small blood vessels, while Group 2 exhibited larger blood vessels spread throughout the remaining pulp tissue not related to inflammatory infiltrate. Both materials behaved histologically similar regarding pulp inflammation and vascularization with intense CD31 positivity on the wall of blood vessels.