The Intestine as a Model for Neuronal Plasticity

Abstract

This study focuses on establishing the nature and extent of the changes that occur in gastroenteric innervation, specifically the myenteric plexus of the rat ileum, following an injury generated by experimental obstruction. A partial obstruction was accomplished by placing a cuff around the terminal portion of the ileum of the rat. Substantial hypertrophy of the enteric muscle wall occurred after 3–5 weeks. Light microscopic examination of the myenteric plexus revealed changes in the numbers of neuronal perikarya, ganglia and perikarya per ganglia; sizes and shapes of perikarya; and thicknesses of nerve fiber bundles. Using vasoactive intestinal polypeptide (VIP) and substance P light microscopic immunohistochemistry, we observed indications of transmitter accumulation in cell bodies and nerve fibers and reactive, degenerative and regenerative changes in axonal endings. Electron microscopic studies provided evidence for neuroplastic changes, as demonstrated by the appearance of reactive and regenerative, or growth, cones in the myenteric plexus. © 1989, International Society of Histology and Cytology. All rights reserved.