OPTIMIZATION AND PRODUCTION OF NEOMYCIN FROM DIFFERENT AGRO INDUSTRIAL WASTES IN SOLID STATE FERMENTATION

Abstract

The cost of neomycin production may be significantly decreased by using inexpensive carbon substrates like agricultural residues. However, scarce information can be found in the literature about the utilization of cellulosic and lignocellulosic residues for obtaining neomycin. Usually agricultural residues producing various toxic compounds to the atmosphere; so, as an interesting alternative to the utilization of agricultural wastes (as apple pomace, cotton seed meal, soy bean powder and wheat bran) for simultaneous neomycin production. The highest neomycin production (2765 µg/g substrate) was achieved with apple pomace in solid-state fermentation. The optimization of physical parameters such as inoculum size, substrate particle size, incubation temperature, initial pH, initial moisture level, incubation period and chemical parameters such as additional carbon and nitrogen sources were studied for the production of neomycin in solid-state fermentation using Streptomyces fradiae NCIM 2418. The optimum values of the critical components determined for the maximum neomycin production were inoculum size 2×106 CFU/g initial dry substrate, substrate particle size 1.2 mm, incubation temperature 30oC, initial pH 8, initial moisture level 70%, fructose (1% w/v), (NH4)2HPO4 (1% w/v), L-glutamine (1%w/v) and incubation period day 10. An overall 2.6-fold improvement in neomycin production was achieved due to optimization.