Identification and preliminary characterization of Vibrio cholerae outer membrane proteins

Abstract

Outer membrane proteins of V. cholerae were purified by sucrose density centrifugation and Triton X-100 extraction at 100 mM Mg2+. The proteins were separated by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. V. cholerae outer membrane proteins presented a unique pattern when compared with the patterns of other gram-negative rods. There were 8 to 10 major bands (M(r) 94,000 to 27,000), with most of the protein located in band 5 (M(r)~45,000), which thus appears to be the major structural protein of the outer membrane. Lipid and carbohydrate were associated with band 6.