Evaluation of gene expression levels in grapevine cultivars after Erysphe necator and methyl jasmonate treatments

Abstract

Abstract: Powdery mildew (PM-Eryshpe necator) is one of the most important diseases threatening the world of viticulture. This study determined that the gene expression levels of Myc2, WRKY1, and DHN1a in Boğazkere (V. vinifera L. PM susceptible), Cabernet Sauvignon (V. vinifera L. PM susceptible), Kishmish Vatkana (V. vinifera L. PM tolerant), and Regent (V. vinifera L. PM tolerant) cultivars after PM and PM-Methyl jasmonate (MeJa) treatments by real-time polymerase chain reaction (RT-PCR). The susceptible and tolerant cultivars that were compared in the study were inoculated with PM which was taken from the natural environment and was sprayed with 50 μM MeJa. Leaf samples of cultivars were collected at 0, 6, 12, 24, 48, 72, 96, and 120 h postinoculation (hpi). RT-PCR analyses of PM and PM-MeJa treatments were performed on WRKY1, DHN1a, and Myc2 genes. Actin1 was used as the reference gene. The analyses concluded that the susceptible and tolerant cultivars utilized different mechanisms and that TF genes were induced in the presence of PM and MeJa. It was determined that PM showed different levels of expression in 3 genes and 4 cultivars. MeJa treatment had a stimulating effect on the response to E. necator infection.