Multidrug resistant and ESBL-producing Salmonella spp. isolated from poultry

Abstract

Salmonella spp. is one of the main agents responsible for foodborne infection in humans, and products of poultry origin are the most common infection sources. Studies have shown the occurrence of antimicrobials resistant Salmonella spp. in animal products. The Extended Spectrum À-Lactamase (ESBL) are enzymes that confer to bacteria the ability to hydrolyze cephalosporin with an oximino side chain and monobactams. This study aimed to investigate antimicrobial resistance profile, identify phenotypes and genotypes for multiple drug resistance (MDR) and that produce ESBL from isolates of Salmonella spp. in the broiler production chain. We used samples of Salmonella spp. (n=11) isolates from poultry, poultry products and poultry-source environment from the state of Maranhao-Brazil. The isolates of Salmonella spp. assessed showed genotypical and phenotypical characteristics of MDR. The results show that 72.72% (08/11) of the strains presented the phenotypic profile for ESBL production. The isolates showed positivity to at least 13.64% (03/22) of the genes studied and the highest frequencies were observed in genes sul1 (73%), dfrA12 (55%), blaCTX-M (55%), tetA, tetB and tetC, with 45%. In conclusion, the strains of Salmonella spp. isolates present genotypic and phenotypic characteristics for MDR and ESBL production, demonstrating the dissemination risk of these microorganisms through the food chain.