Anti-Cancer activity of liposomal medical leech saliva extract (LSE)

Abstract

Leech could be a trendy therapeutic agent introduced by Food and Drug Administration (FDA) and contains totally different peptides and proteins as well as histamine, serotonin, steroid hormones and modulators, enzymes, proteinase inhibitors and anti-microbial agent. human breast carcinoma cell line (MCF-7) was utilized in this project. Medical Leech saliva (Hirudo Medicinalis) extracted with %8 ethanol in deionized water without leech scarification. Demonstrated the biological activity of Leech Saliva Extract (LSE) as Anti-Cancer agent in vitro, the MCF-7 cell line was cultured in RPMI that has 10% fetal bovine blood serum (FBS) at 37°C with 5% CO2. Cell line divided into 2 groups A and B. Type A simply treated with liposomal LSE and group B treated with leech saliva without liposomal formulation. For testing Anti-Cancer activity of liposomal LSE serial dilution of solutions were added to cell line. LDH test was utilized in this study to the determined cytotoxic activity of components and for showing anti-cancer activity Scratching test was used and Apoptotic bodies were determined by DAPI staining. Results demonstrated that Liposomal LSE as a targeting drug has the more practical action against LSE on MCF-7 cell line. SDS-PAGE of LSE showed that it contains about hundred proteins. Every of those proteins have own activity in body. Treatment Graphs verified that formulation of liposomal LSE has a lot of impact on Cancer cells.