Evaluation of droplet digital PCR for the clinical diagnosis of toxoplasmosis

  • Recalt E
  • Lespagnol A
  • Hamet L
  • et al.
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Abstract

To date, droplet digital PCR (ddPCR) contributes to the detection of infectious agents in the environment, but few studies have evaluated its interest in human diagnosis, particularly the Naica Crystal ddPCR. This study shows that it has similar performance to real-time PCR for the diagnosis of toxoplasmosis and could be of interest for parasite quantification, but its cost limits its use.

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Recalt, E., Lespagnol, A., Hamet, L., Guegan, H., Gangneux, J.-P., Galibert, M.-D., & Robert-Gangneux, F. (2025). Evaluation of droplet digital PCR for the clinical diagnosis of toxoplasmosis. Microbiology Spectrum, 13(12). https://doi.org/10.1128/spectrum.02984-25

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