Fluorescent labeling of peptides on solid phase

Abstract

N α-Fmoc-Nε-[(7-methoxycoumarin-4-yl) acetyl]-l-lysine (Nα-Fmoc-l-Lys(Mca)-OH) 3 is conveniently prepared by benzotriazole methodology (52% over two steps). N-Acylbenzotriazoles Mca-Bt 2, Nα-Fmoc-l-Lys(Mca)-Bt 4, coumarin-3-ylcarbonyl (Cc)-Bt 5, Nα-Fmoc-l-Lys(Cc)-Bt 7 and Nα-(Cc)- l-Lys(Fmoc)-Bt 9 enable the efficient microwave enhanced solid-phase fluorescent labeling of peptides. © 2008 The Royal Society of Chemistry.