Expression of Envelope Protein (E2) of Bovine Viral Diarrhea Virus in Insect Cells

Abstract

The gene encoding the envelope glycoprotein (E2) of bovine viral diarrhea virus (BVDV) was expressed in a baculovirus. The expressed protein was detected on the surface of infected cells by immunofluorescence. Western blotting analysis showed the presence of the expressed protein of a similar molecular size to the E2 protein. The antigenicity of expressed protein were tested in guinea pigs and cattle. The immunized animals developed neutralizing antibodies against BVDV.