Study of Acetobacter xylinum FNCC 0001 fermentation kinetics using artificial media containing various carbon and nitrogen concentrations

Abstract

The starter medium's proper composition was needed to obtain the best bacterial growth medium. Therefore, coconut water artificial medium was used with 3 variations of glucose and peptone concentrations as carbon and nitrogen sources respectively to obtain the best formulation in Acetobacter xylinum FNCC 1 fermentation kinetics. Both concentrations' effects were evaluated using the fermentation kinetics parameter. The growth curve, bacterial cellulose (BC) yield, pH change, and fermentation kinetics were observed at 24 points within 7 days. Furthermore, the variations were 6.612 g/L glucose and 16 g/L peptone (F1), 9.541 g/L glucose and 21 g/L peptone (F2), and 17.172 g/L glucose and 26 g/L peptone (F3). The results showed that carbon and nitrogen concentrations' variations affected the bacterial growth and fermentation kinetics. The F1 treatment gave the best fermentation kinetics based on the best cell growth kinetics parameters including specific growth rate (Rx or μ), doubling time (Td), multiplication degree (n), and maximum specific growth rate (μ max).