Expression and Function of Allergin-1 on Human Primary Mast Cells

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Abstract

Mast cells (MC) play an important role in allergic and non-allergic immune responses. Activation of human MC is modulated by several cell surface inhibitory receptors, including recently identified Allergin-1 expressed on both human and mouse MC. Although Allergin-1 suppresses IgE-mediated, mast cell-dependent anaphylaxis in mice, the expression profile and function of Allergin-1 on human primary MC remains undetermined. Here, we established a seven-color flow cytometry method for assessing expression and function of a very small number of human primary MC. We show that Allergin-1S1, a splicing isoform of Allergin-1, is predominantly expressed on human primary MC in both bronchoalveolar lavage (BAL) fluid and nasal scratching specimens. Moreover, Allergin-1S1 inhibits IgE-mediated activation from human primary MC in BAL fluid. These results indicate that Allergin-1 on human primary MC exhibits similar characteristics as mouse Allergin-1 in the expression profile and function. © 2013 Nagai et al.

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Nagai, K., Tahara-Hanaoka, S., Morishima, Y., Tokunaga, T., Imoto, Y., Noguchi, E., … Shibuya, A. (2013). Expression and Function of Allergin-1 on Human Primary Mast Cells. PLoS ONE, 8(10). https://doi.org/10.1371/journal.pone.0076160

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