Corrosion and biocompatibility behaviours of microarc oxidation/phytic acid coated magnesium alloy clips for use in cholecystectomy in a rabbit model

Abstract

With the popularisation of laparoscopic cholecystectomy, ligation clips have been commonly used for ligating the cystic duct and cystic artery. However, non-degradable clips remain in the body long-term, which significantly increases the risk of the clip becoming detached. Thus, magnesium alloys have attracted tremendous attention owing to their biodegradability and good biocompatibility. However, the poor corrosion resistance hinders the clinical application of magnesium alloys with microarc oxidation/phytic acid (MAO/PA) composite coatings as protective coatings. Here, these alloys were used to hinder the rapid material degradation in aqueous solution. Electrochemical tests were conducted to evaluate thein vivodegradation behaviour in simulated body fluid (SBF) for Mg-Zn-Y-Nd alloys, and scanning electron microscopy (SEM) was used to observe the micromorphology ofin vivoclip degradation. Cell toxicity, cell adhesion, and flow cytometry were performedin vitroto detect cytocompatibility. Biochemical detection of serum magnesium, serum creatinine (CREA), blood urea nitrogen (BUN), alanine transaminase (ALT), and alanine aminotransferase (AST), and haematoxylin-eosin (HE) staining of the heart, liver, and kidney tissuesin vivowas conducted to determine the biocompatibility properties after surgery. Electrochemical measurements and SEM images revealed that the MAO/PA-coated magnesium alloy delayed corrosion in SBF. The apoptosis rate increased slightly with increased extract concentration. Nevertheless, MAO/PA-coated magnesium alloys still exhibited good cytocompatibility. No obvious abnormality was observed in the blood biochemical test or HE staining. Thus, MAO/PA-coated magnesium alloys exhibit better corrosion than bare magnesium. In addition, Mg-Zn-Y-Nd and MAO/PA-coated magnesium alloys exhibited no cytotoxicity, good adhesion, and biosafety.