A test of the relationship between sequence and structure in proteins: Excision of the heme binding site in apocytochrome b5

Abstract

The water-soluble domain of rat hepatic holocytochrome b5 is an αβ protein containing elements of secondary structure in the sequence β1-α1- β4-β3-α2-α3-β5-α4-α5-β2-α6. The heme group is enclosed by four helices, α2, α3, α4, and α5. To test the hypothesis that a small b hemoprotein can be constructed in two parts, one forming the heme site, the other an organizing scaffold, a protein fragment corresponding to β1-α1- β4-β3-Λ-β2-α6 was prepared, where Λ is a seven-residue linker bypassing the heme binding site. The fragment ('abridged b5') was found to contain α and β secondary structure by circular dichroism spectroscopy and tertiary structure by Trp fluorescence emission spectroscopy. NMR data revealed a species with spectral properties similar to those of the full-length apoprotein. This folded form is in slow equilibrium on the chemical shift time scale with other less folded species. Thermal denaturation, as monitored by circular dichroism, absorption, and fluorescence spectroscopy, as well as size-exclusion chromatography-fast protein liquid chromatography (SEC-FPLC), confirmed the Coexistence of at least two distinct conformational ensembles. It was concluded that the protein fragment is capable of adopting a specific fold likely related to that of cytochrome b5, but does not achieve high thermodynamic stability and cooperativity. Abridged b5 demonstrates that the spliced sequence contains the information necessary to fold the protein. It suggests that the dominating influence to restrict the conformational space searched by the chain is structural propensities at a local level rather than internal packing. The sequence also holds the properties necessary to generate a barrier to unfolding.