Studies of chlorophyll content by different methods in black gram (Vigna mungo L.)

Abstract

Extraction, estimation and determination of different pigments from black gram leaves by different methods have been evaluated. The main pigments are chlorophyll a, b and phaeophytin content are studied at different wave lengths. Several methods are used for this purpose but a simple and easy incubation method dispensing grinding and centrifugation procedures is described. The recovery of chlorophyll pigments by incubation method in which tender leaf tissue in 80% buffered acetone at 4°C give higher yield of pigments compared to other methods. The use of acetone to extract chlorophyll by incubation is found to be superior to methods in extraction of pigments. © 2007 Academic Journals.