Investigation of Nematopsis spp. oocysts in 7 species of bivalves from Chonburi Province, Gulf of Thailand

Abstract

This is the first detailed report of Nematopsis spp. in Thai bivalves. A monthly survey was conducted on 7 species of commercial bivalves from Chonburi province, on the eastern seaboard of Thailand, from November 2000 to November 2001 to investigate the prevalence of the apicomplexan parasite Nematopsis Schneider, 1892. Nematopsis spp. sporozoites were found in the cultivated bivalves Aicuatula arcuatula, Anadara granosa and Perna viridis as well as the locally harvested Paphia undulata. They were not found in Donax faba, Meretrix meretrix or Saccostrea cucullata. Using light microscopy, we were able to identiby 4 oocyst morphotypes of the gregarine Nematopsis spp. Prevalence of Nematopsis spp. during the 13 mo sampling period was highest in A. arcuatula (91.8%; n = 110) and lowest in A. granosa (59.2%; n = 130). The morphology of the oocysts differed between hosts, with an average (x ± SD) length/width of 16.28 ± 0.64/12.01 ± 0.35 μm (n = 50) for A. arcuatula, 16.90 ± 0.71/12.69 ± 0.33 μm (n = 50) for A. granosa, 17.61 ± 0.69/12.72 ± 0.36 μm (n = 50) for P. viridis, and 11.21 ± 0.62/8.55 ± 0.52 μm (n = 50) for P. undulata. Identification of oocysts of these apicomplexan gregarines to species was not attempted. The prevalence of infection in relation to habitat and time of sampling is discussed.