Partition of proteases from Lentinus citrinus DPUA 1535 by the peg/phosphate aqueous two-phase system

Abstract

A full two-level factorial design was employed to study the influence of PEG molar mass (MMPEG), PEG concentration (CPEG) and phosphate concentration (CPHOSPH) on proteases partition by Lentinus citrinus DPUA 1535 in a PEG/phosphate aqueous two-phase system (ATPS). For all ATPS studied, proteases partitioned for the top phase and the best proteases extraction condition was obtained with MMPEG = 6000 g mol -1, CPEG = 17.5% (w/w) and CPHOSPH = 25% (w/w) with (1.1) purification factor and (151%) activity yield. Findings reported here demonstrate a practical strategy that serves as a first step for proteases purification from crude extract by L. citrinus.