Freeze-fracture of membrane fusions during exocytosis in pancreatic B-cells

Abstract

To examine the freeze-fracture appearance of membrane alterations at sites of exocytosis in mammalian cells, the authors studied the secretory granule and plasma membrane of rat pancreatic B-cells during glucose-stimulated insulin secretion. Constant features observed were the scarcity of particles in secretory-granule P-fracture faces and the almost total clearance of intramembranous particles in P- and E-fracture faces of the plasma membrane in areas of close apposition of these two membranes preceding fusion; also observed was the temporary persistence of particle-cleared regions after the fusion was completed. These observations thus support the concept that membranes fuse at sides of closely apposed, particle-free regions and that the physiologically created clear areas found in freeze-fracture replicas of the plasma membrane are the hallmarks of incipient or recent membrane fusion.