Differential contribution of BLTand BLTto leukotriene BInduced human NK cell cytotoxicity and migration

Abstract

Accumulating evidence indicates that leukotriene B(LTB via its receptors BLTand/or BLT(BLTRs) could have an important role in regulating infection, tumour progression, inflammation, and autoimmune diseases. In the present study, we showed that LTBnot only augments cytotoxicity by NK cells but also induces their migration. We found that approximately 30% of fresh NK cells express BLT 36% express BLT and 15% coexpress both receptors. The use of selective BLTR antagonists indicated that BLTwas involved in both LTBinduced migration and cytotoxicity, whereas BLTwas involved exclusively in NK cell migration, but only in response to higher concentrations of LTB BLTand BLTexpression increased after activation of NK cells with IL-2 and IL-15. These changes of BLTR expression by cytokines were reflected in enhanced NK cell responses to LTB Our findings suggest that BLTand BLTplay differential roles in LTBinduced modulation of NK cell activity.