Cell-Surface Protein Labeling with Luminescent Nanoparticles through Biotinylation by Using Mutant β-Lactamase-Tag Technology

Abstract

Time-lapse fluorescence imaging: We have developed a novel biotinylation probe, BHA, based on mutant β-lactamase tag (BL-tag) technology for tethering target proteins and streptavidin-conjugated luminescent nanoparticles. This method enabled visualization of BL-tag-fused epidermal growth factor receptor (EGFR) internalization and pulse-chase labeling by using three kinds of quantum dots (QDs). © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.