Genome Editing in Mice Using CRISPR/Cas9: Achievements and Prospects

Abstract

History Animal models of diseases Animal models, both vertebrates and invertebrates have been instrumental for dissecting the pathophysiology of human diseases. Despite divergent opinions over their use, animal models remain the most powerful tools to understand the mechanisms underlying physiological processes, and their pathological counterparts. They are also invaluable tools to search for disease modifiers and to develop and test novel treatment strategies. Although each model has intrinsic limitations, the use of animals as an entire systemic model is vital to biomedical research because they address metabolic and physiologic processes, which cannot be studied in isolated tissue culture.