STUDY OF ANGIOGENIC POTENTIAL OF MORINGA OLEIFERA LAM. ON CHICKEN CHORIOALLANTOIC MEMBRANE (CAM)

Abstract

Angiogenesis plays an important role in many physiological and pathological conditions. Currently, the drugs possessing pro-angiogenic property as compared to anti-angiogenic property are limited. The primary objective of study was to find the angiogenic potential of hot methanolic extract of leaves of Moringa Oleifera Lam. The hot methanolic extract of moringa leaves was prepared using soxhlet extraction. Phytochemical analysis was done by thin layer chromatography, ultra violet and infrared spectrophotometer. The egg candling instrument was prepared indigenously to observe the angiogenesis in Chick Chorioallantoic Membrane (CAM) model of hen’s egg. The eggs obtained from Aarey Poultry, Goregaon (Mumbai) were observed for their fertility and Embryonic Development Day (EDD). The growth of embryo was observed every day till 7th day of EDD. The test sample was loaded on 8th day and the angiogenic effect was observed and photographed every day till 11th day. The PlerminTM ointment (ββ PDGF) (Platelet Derived Growth Factor) was used as positive control. Quantification of blood vessels was done with growth index [(No of BLVS11th day – no of blood vessels 8th day)/ No of BLVS11th day], Abgel© sponge assay and compared with PDGF.