A first genotyping assay of French cattle breeds based on a new allele of the extension gene encoding the melanocortin-1 receptor (Mc1r)

Abstract

The seven transmembrane domain melanocortin-1 receptor (Mc1r) encoded by the coat colour extension gene (E) plays a key role in the signalling pathway oof melanin synthesis. Upon the binding of agonist (melanocortin hormone, α-MSH) or antagonist (Agouti protein) ligans, the melanosomal synthesis of eumelanin and/or phaeomelanin pigments is stimulated or inhibited, respectively. Different alleles of the extension gene were clones from unrelated animals belonging to French cattle breeds and sequencing. The wild type E allele was mainly present in Normande cattle, the dominant ED allele in animals with black color (i.e. Holstein), whereas the recessive e allele was identified in homozygous animals exhibiting a more or less strong red coat color (Blonde d'Aquitaine, Charolaise, Limousine and Salers). A new allele, named E1, was found in either homozygous (E1/E1) or heterzygous individuals (E1/E) in Aubrac and Gasconne breeds. This allele displayed a 4 amino acid duplication (12 nucleotides) location within the third cytoplasmic loop of the receptor,, a region known to interact with G proteins. A first genotyping assay of the main French cattley breeds is described based on these four extensio alleles.