Bacterial collagen-templated synthesis and assembly of inorganic particles

Abstract

Collagen has been used as a common template for mineralization and assembly of inorganic particles, because of the special arrangement of its fibrils and the presence of charged residues. Streptococcal bacterial collagen, which is inherently secreted on the surface of Streptococcus pyogenes, has been progressively used as an alternative for type I animal collagen. Bacterial collagen is rich in charged amino acids, which can act as a substrate for the nucleation and growth of inorganic particles. Here, we show that bacterial collagen can be used to nucleate three different inorganic materials: hydroxyapatite crystals, silver nanoparticles, and silica nanoparticles. Collagen/mineral composites show an even distribution of inorganic particles along the collagen fibers, and the particles have a more homogenous size compared with minerals that are formed in the absence of the collagen scaffold. Furthermore, the gelation of silica occurring during mineralization represents a means to produce processable self-standing collagen composites, which is challenging to achieve with bacterial collagen alone. Overall, we highlight the advantage of simply combining bacterial collagen with minerals to expand their applications in the fields of biomaterials and tissue engineering, especially for bone regenerative scaffolds.