Association of interleukin 4-C590T gene polymorphism with nephrotic syndrome in children

Abstract

Nephrotic syndrome is one of common glomerular disorder in children.Response to steroid therapy maybe associated with interleukin 4 promoter site gene polymorphisms.The aim is to investigate the role of IL-4 - gene polymorphisms in disease predisposition. Sixty patients of nephrotic syndrome (30 of them were steroid sensitive and the other 30 Patients were steroid resistant)with thirty apparently healthy individuals as control group were participated in this study. Blood samples were collected from patients and controls and DNA extraction was made from each blood sample then single nucleotide gene polymorphisms were investigated by using restriction fragment length polymorphism technique (RFLP) and ELISA technique was used to detect the serum level of IL-4.The heterozygous genotype (CT) of IL-4 C590T gene polymorphism was slightly more frequent among patients than controls without significant difference. Serum levels of IL-4 among patients show elevated values as compared to control group.IL-4 may affect response to the steroid therapy in nephrotic syndrome patients.