A platform to view huntingtin exon 1 aggregation flux in the cell reveals divergent influences from chaperones hsp40 and hsp70

Abstract

Background: How misfolded proteins such as mutant huntingtin aggregate in the cell remains enigmatic. Results:Webuilt a platform to view how aggregation proceeds and assessed the impact of quality control chaperones hsp40 and hsp70. Conclusion: hsp70 enhanced survival of cells with aggregates; hsp40 suppressed aggregation. Significance: We developed a new toolkit to illustrate the impact of protein aggregation on cell biology. © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.