Optimization of biomass production by ustilago tnaydis in submerged culture using Taguchi experimental design

Abstract

The aim of this study was to optimize the submerged culture conditions in baffled Erlenmeyer flasks for biomass production by U. maydis FBI 2 by L 8 Taguchi orthogonal array experimental design using YPD broth (yeast extract-peptone-dextrose) as a basal medium. The optimized conditions (glucose 40 g L-1, [Cu] 0.005 g L-1, pH 7, orbital shaking speed 200 rpm, temperature 32°C) predicted a final biomass concentration of 13.88 g L-1 after 48 h. When these conditions were tested experimentally, a real final biomass concentration of 15.67 g L-1 was obtained and the microorganism presented a specific growth rate μ = 0.03 h-1. © 2008 Asian Network for Scientific Information.