Complete Genome Sequence of Bacillus mycoides TH26, Isolated from a Permafrost Thaw Gradient

Abstract

Here, we describe the complete genome assembly of a Bacillus mycoides isolate collected from a boreal forest soil associated with permafrost thaw. Using only long-read Nanopore sequences and VolTRAX library preparation, we assembled two circular contigs totaling 5,789,722 bp ( N 50 , 5,306,036), a complete chromosome with one plasmid. Here, we describe the complete genome assembly of a Bacillus mycoides isolate collected from a boreal forest soil associated with permafrost thaw. Using only long-read Nanopore sequences and VolTRAX library preparation, we assembled two circular contigs totaling 5,789,722 bp ( N 50 , 5,306,036), a complete chromosome with one plasmid.