Dual role of BAR domain-containing proteins in regulating vesicle release catalyzed by the GTPase, dynamin-2

Abstract

Background: Membrane curvature generation is essential for dynamin-2-catalyzed membrane fission and vesicle release. Results: Dynamin-2 binding partners with curvature generating activity differentially regulate the assembly, GTPase, and fission activities of dynamin-2. Conclusion: Dynamin-2 partners display complex patterns of regulation. Significance: The distinct functional interactions between dynamin-2 and its binding partners position them to contribute to the spatio-temporal regulation of clathrin-mediated endocytosis. © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.