A single nucleotide polymorphism at the taqman probe-binding site impedes real-time reverse transcription-PCR-based detection of norovirus GII.4 Sydney

Abstract

A single nucleotide polymorphism (SNP) in the TaqMan probe-binding site resulted in decreased sensitivity of real-time reverse transcription-PCR (RT-rtPCR) for detection of norovirus genogroup II genotype 4 (GII.4) Sydney. A new degenerate probe was designed that improved the sensitivity of the detection while not interfering with the detection of other GII and GI strains.