Perda de amostras em tissue mkroarray: Comparação entre técnicas com uso de fita adesiva comercial, lâminas silanizadas pelo método traditional ou por método modificado

Abstract

The tissue mkroarray (TMA) technique allows the evaluation of multiple tissue samples in a single block. One of the problems of TMA is the ungluing of tissue sections, thus commercial adhesive tape has been used to reduce this loss. There are no reports comparing the use of the commercial adhesive tape with the use of the modified silane-coated technique. The objective of this study was to compare section loss in slides using commercial adhesive tape, silane-coated microslides with the conventional technique or with the modified technique. Material and method: The TMA was constructed with hepatic tissue blocks embedded in paraffin, using a fixed base device, placing 32 cylinders of 2 mm in diameter in duplicate and 2.2 mm apart from each other. Fifteen 4-μm sections were placed on conventional silane-coated microslides at 4% (Croup 1), 15 on silane