LncRNA TP73-AS1 interacted with MIR-141-3p to promote the proliferation of non-small cell lung cancer

Abstract

Introduction: Recent studies have shown that long non-coding RNAs (lncRNAs) are involved in a variety of biological processes and diseases in humans, including cancer. However, the exact effects and molecular mechanisms of TP73-AS1 in non-small cell lung cancer (NSCLC) progression are still unknown. The present study is aimed to reveal the detailed functions and the mechanism of TP73-AS1 in the regulation of NSCLC cell proliferation. Material and methods: TP73-AS1 expression in NSCLC tissues and cell lines was determined using real-time PCR assays. The functions of TP73-AS1 in the regulation of NSCLC cell proliferation was evaluated using BrdU assays. The interaction between TP73-AS1 and MIR-141-3p was confirmed using luciferase report gene assays. Results: TP73-AS1 was upregulated in NSCLC tissues and cell lines. However, when knockdown of TP73-AS1 inhibited the NSCLC proliferation. By using online tools, we screened out MIR-141-3p may combined with TP73-AS1. With use of luciferase assays, we confirmed that MIR-141-3p could directly bind to TP73-AS1. In NSCLC tissues, MIR-141-3p was down-regulated; TP73-AS1 was inversely correlated with MIR-141-3p. Conclusions: Our data suggest that TP73-AS1 might be an oncogenic lncRNA that promotes proliferation of NSCLC and might be regarded as a therapeutic target in NSCLC.