Biocompatibility and anti-bacterial activity of Zn-containing HA/TiO2 hybrid coatings on Ti substrate

Abstract

To improve the osteogenic and anti-bacterial activity for the hydroxyapatite coated titanium substrate Zn containing HA biological coating was prepared on commercially pure Ti via the sol-gel method after treatment by oxidation which produced a porous titania coating on the titanium. The phase structures of the coatings were characterized by X-ray diffraction. The release concentration of zinc was measured by an inductively coupled plasma spectrometer. In vitro co-culture assays were performed to evaluate the cell morphology and attachment of MG-63 cells to the coated titanium. The anti-bacterial effect of the ZnHA/TiO2 coating was evaluated on Porphyromonas gingivalis w83. The cell micro morphology on the ZnHA/TiO2 coatings was more polygonal than that of HA/TiO2 coatings when viewed by SEM. It was confirmed by fluorescence microscopy observations that the MG-63 cells on the hybrid coating layer were favorably adhered. Compared with the HA/TiO2 coating, the ZnHA/TiO2 coating significantly inhibited the growth of Porphyromonas gingivalis. The results suggest that the ZnHA/TiO2 hybrid coatings on titanium implants should possess better mechanical binding properties and biocompatibility. © 2013 The Hard Tissue Biology Network Association.